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This heat induced decrease in 1,2 DAG level in WRK 1 cells was not due to an increase in turnover rate of 1,2 DAG to phosphatidic acid (PA) since the decrease in 1,2 DAG was not affected when cells were heated in the presence of die DAG kinase inhibitor, dioctanoylethylene glycol (diC(_8)EG). The increase in TAG level may be due to a rapid steroids, he at induced increase in TAG synthesis from 1,2 DAG, thus leading to decreased levels of 1,2 DAG. Heat also led to an increase in inositol bisphosphate (InsP(_2)) and inositol trisphosphate (InsP(_3)) but not inositol monophosphate (InsP(_1)) or higher inositol phosphate (InsP(_4/5/6)) levels in WRK 1 cells.
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